A case study of a typical office room was carried out in UTM campus in Johor Bahru with field measurement conducted for 15 days in naturally ventilated office space. The effectiveness of the thermal performance of single-sided ventilated room is been validated in order to mark out the maximum range of temperature and relative humidity in a single-sided ventilation room. This paper discussed the study of thermal performance of the single-sided ventilation room by comparing two simulation software results and field experiment result. However, due to limitation of layout efficiency, cost effectiveness, security, and fire requirement, most of the building designed has single-sided ventilation solution. Cross ventilation strategy is considered as the ideal in order to enhance the thermal performance. An effective passive design strategy for building is necessary especially in hot and humid climate in Malaysia. In Malaysia, 70% of the electricity consumed is for cooling load. In order to reduce energy consumption, effectiveness of natural ventilation performance in building should become major consideration in design. Ventilation is one of the important criteria to achieve thermal comfort in building.
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